Exhibit 8.1

Kitov Pharmaceuticals Holdings Ltd.

The following table sets forth the name and jurisdiction of incorporation of our subsidiaries as of the date hereof.

*On April 25, 2017, the boards of directors of each of Kitov Pharmaceuticals Holdings Ltd. and Kitov Pharmaceuticals Ltd. approved a merger between the two entities, with Kitov Pharmaceuticals Holdings Ltd.remaining as the surviving entity. For more information on the proposed merger, see Item 4.C – Organizational Structure in the Annual Report for 2016 on Form 20-F in which this is Exhibit is included.